Priming immunization against cholera toxin and E. coli heat-labile toxin by a cholera toxin short peptide-beta-galactosidase hybrid synthesized in E. coli.
نویسندگان
چکیده
منابع مشابه
The ADP-ribosyltransferase activity of cholera toxin and Escherichia coli heat-labile toxin.
Takai, Y., Kishimoto, A,, Kawahara, Y., Minakuchi, R.. Sano, K., Kikkawa, U., Mori, T., Yu, B., Kaibuchi, K. & Nishizuka, y. (1981) Adv. Cyclic Nucleotide Res. 14, 301-313 Thomas, D. D. & Knoop, F. C. (1982) J. Inject Dis. 145, 141147 Thomas, D. D. & Knoop, F. C. (1983) J . Infect. Dis. 147,450-459 Turnberg, L. A., Bieberdorf, F. A,, Morawski, S. G . & Fordtran, J. S. (1970) J. Clin. Invest. 49...
متن کاملFusion of Cholera toxin B subunit (ctxB) with Shigella dysenteriae type I toxin B subunit (stxB), Cloning and Expression that in E. coli
Background and Objective: Shiga toxin (STx) is the main virulence factor in Shigella Dysenteriae type I and is composed of an enzymatic subunit STxA monomer and a receptor-binding STxB homopentamer. Shigella toxin B subunit (STxB) is a non-toxic homopentameric protein responsible for toxin binding and internalization into target cells by interacting with glycolipid (Gb3). Cholera toxi...
متن کاملEvaluation and optimization of a latex agglutination assay for detection of cholera toxin and Escherichia coli heat-labile toxin.
The effectiveness of a latex agglutination assay kit for the detection of Escherichia coli heat-labile toxin and cholera toxin was determined for the identification of natural isolates of the corresponding enteric pathogens in Southeast Asia. By selection of the appropriate culture media, the sensitivity of the assay was improved from 90.6% (for the detection of heat-labile toxin) and 75% (for ...
متن کاملTargeting of cholera toxin and Escherichia coli heat labile toxin in polarized epithelia: role of COOH-terminal KDEL
Vibrio cholerae and Escherichia coli heat labile toxins (CT and LT) elicit a secretory response from intestinal epithelia by binding apical receptors (ganglioside GM1) and subsequently activating basolateral effectors (adenylate cyclase). We have recently proposed that signal transduction in polarized cells may require transcytosis of toxin-containing membranes (Lencer, W. I., G. Strohmeier, S....
متن کاملCharacterization of the receptor for cholera toxin and Escherichia coli heat-labile toxin in rabbit intestinal brush borders.
125I-labelled heat-labile toxin (from Escherichia coli) and 125I-labelled cholera toxin bound to immobilized ganglioside GM1 and Balb/c 3T3 cell membranes with identical specificities, i.e. each toxin inhibited binding of the other. Binding of both toxins to Balb/c 3T3 cell membranes was saturable, with 50% of maximal binding occurring at 0.3 nM for cholera toxin and 1.1 nM for heat-labile toxi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1985
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1985.tb04086.x